This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD58 Antibody (Biotin) LS-C35694
catalog :
LS-C35694
clonality :
monoclonal
host :
mouse
conjugate :
biotin
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
product information
AntibodyID :
35202
AntibodyName :
LS-C35694
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CD58 Antibody (Biotin) LS-C35694
Specificity :
Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.
ClonalityDesc :
Monoclonal
AntibodyModification :
Biotin
PresentationDesc :
PBS, pH 7.2, 0.05% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenDesc :
Human cytotoxic T cells.
PurificationDesc :
Protein A purified
IsotypeName :
IgG1
Gene :
CD58
StandardGeneSymbol :
CD58
gene family :
Immunoglobulin
Reactivity :
Human
Usage :
Ctox, Flo, IHC, IHC-Fr, IP
ShortWebDescription :
CD58 antibody LS-C35694 is a biotin-conjugated mouse monoclonal antibody to human CD58. Validated for Ctox, Flow, IHC and IP.
UsageText :
Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 ml of anti-CD58/Biotin at 10 mg/ml. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94% blocked when pre incubated 10 minutes with 20 ml of 0.5 mg/ml anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 molecule
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.