product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
TH / Tyrosine Hydroxylase Antibody (N-Terminus) LS-C354110
catalog :
LS-C354110
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1
Reference
Xiong L, Liu Y, Zhou M, Wang G, Quan D, Shuai W, et al. Targeted ablation of cardiac sympathetic neurons attenuates adverse postinfarction remodelling and left ventricular dysfunction. Exp Physiol. 2018;103:1221-1229 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C354110 image 1
Western blot analysis of Tyrosine Hydroxylase expression in DLD (A); mouse liver (B); PC12 (C) whole cell lysates.
image 2 :
LifeSpan Biosciences LS-C354110 image 2
Immunohistochemical analysis of Tyrosine Hydroxylase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
image 3 :
LifeSpan Biosciences LS-C354110 image 3
Immunofluorescent analysis of Tyrosine Hydroxylase staining in DLD cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
AntibodyID :
365231
AntibodyName :
LS-C354110
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
TH / Tyrosine Hydroxylase Antibody (N-Terminus) LS-C354110
Specificity :
Recognizes endogenous levels of Tyrosine Hydroxylase protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Tyrosine Hydroxylase.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Gene :
TH / Tyrosine Hydroxylase
BlockingPeptide :
LS-E42542
StandardGeneSymbol :
TH
gene family :
Monooxygenase
Reactivity :
Mouse, Dog, Bovine, Rat, Human
Usage :
ICC (1:100 - 1:500), IF, IHC, IHC-P (1:100 - 1:200), IP (1:10 - 1:100), WB (1:500 - 1:1000)
ShortWebDescription :
Tyrosine Hydroxylase antibody LS-C354110 is an unconjugated rabbit polyclonal antibody to Tyrosine Hydroxylase (TH) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Cited in 1 publication.
Synonyms :
TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.