CTTN / Cortactin Antibody (phospho-Tyr421) LS-C353975 | LifeSpan Biosciences LS-C353975 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

CTTN / Cortactin Antibody (phospho-Tyr421) LS-C353975

catalog :

LS-C353975

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 2

Reference
Stock K, Borrink R, Mikesch J, Hansmeier A, Rehkämper J, Trautmann M, et al. Overexpression and Tyr421-phosphorylation of cortactin is induced by three-dimensional spheroid culturing and contributes to migration and invasion of pancreatic ductal adenocarcinoma (PDAC) cells. Cancer Cell Int. 2019;19:77 pubmed publisher
Zhu L, Cho E, Zhao G, Roh M, Zheng Z. The Pathogenic Effect of Cortactin Tyrosine Phosphorylation in Cutaneous Squamous Cell Carcinoma. In Vivo. 2019;33:393-400 pubmed publisher

image

image 1 :

Western blot analysis of Cortactin (pY421) expression in HeLa UV-treated (A); NIH3T3 H2O2-treated (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Cortactin (pY421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

product information

AntibodyID :

365096

AntibodyName :

LS-C353975

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

CTTN / Cortactin Antibody (phospho-Tyr421) LS-C353975

Specificity :

Recognizes endogenous levels of Cortactin (pY421) protein.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

pTyr421

PresentationDesc :

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

ImmunogenType :

Synthetic peptide - KLH conjugated

ImmunogenDesc :

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Cortactin (pY421).

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Gene :

CTTN / Cortactin

BlockingPeptide :

LS-E42375

StandardGeneSymbol :

CTTN

Reactivity :

Mouse, Human

Usage :

IHC, IHC-P (1:100 - 1:200), IP (1:10 - 1:100), WB (1:500 - 1:1000)

ShortWebDescription :

Cortactin antibody LS-C353975 is an unconjugated rabbit polyclonal antibody to Cortactin (CTTN) (pTyr421) from human. It is reactive with human and mouse. Validated for IHC, IP and WB. Cited in 1 publication.

Synonyms :

CTTN, Cortactin, EMS1, Oncogene EMS1, Amplaxin, Src substrate cortactin

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.