product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
SMG7 Antibody (Internal) LS-C353204
catalog :
LS-C353204
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
more info or order :
citations: 1
image
image 1 :
Western blot analysis of SMG7 expression in Jurkat (A); A431 (B); HeLa (C) whole cell lysates.
image 2 :
Immunohistochemical analysis of SMG7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of SMG7 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
AntibodyID :
364325
AntibodyName :
LS-C353204
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
SMG7 Antibody (Internal) LS-C353204
Specificity :
Recognizes endogenous levels of SMG7 protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human SMG7.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Gene :
SMG7
BlockingPeptide :
LS-E41561
StandardGeneSymbol :
SMG7
Reactivity :
Human
Usage :
ICC (1:100 - 1:500), IF, IHC, IHC-P (1:100 - 1:200), IP (1:10 - 1:100), WB (1:500 - 1:1000)
ShortWebDescription :
SMG7 antibody LS-C353204 is an unconjugated rabbit polyclonal antibody to human SMG7 (Internal). Validated for ICC, IF, IHC, IP and WB.
Synonyms :
SMG7, EST1-like protein C, EST1C, Ever shorter telomeres 1C, HSMG-7, SGA56M, SMG-7, C1orf16, KIAA0250, Protein SMG7, SMG-7 homolog
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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