product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
TGFB1 / TGF Beta 1 Antibody (C-Terminus) LS-C352937
catalog :
LS-C352937
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 1
image
image 1 :
Western blot analysis of TGF beta 1 expression in HeLa (A); MCF7 (B); SP2/0 (C); H9C2 (D) whole cell lysates.
image 2 :
Immunohistochemical analysis of TGF beta 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of TGF beta 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
AntibodyID :
364058
AntibodyName :
LS-C352937
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
TGFB1 / TGF Beta 1 Antibody (C-Terminus) LS-C352937
Specificity :
Recognizes endogenous levels of TGF beta 1 protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human TGF beta 1.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Gene :
TGFB1 / TGF Beta 1
BlockingPeptide :
LS-E43577
StandardGeneSymbol :
TGFB1
gene family :
TGF beta
Reactivity :
Mouse, Dog, Sheep, Bovine, Rat, Pig, Human
Usage :
ICC (1:100 - 1:500), IF, IHC, IHC-P (1:100 - 1:200), IP (1:10 - 1:100), WB (1:500 - 1:1000)
ShortWebDescription :
TGF Beta 1 antibody LS-C352937 is an unconjugated rabbit polyclonal antibody to TGF Beta 1 (TGFB1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Cited in 1 publication.
Synonyms :
TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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