This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CPE / Carboxypeptidase E Antibody (aa42-453) LS-C35273
catalog :
LS-C35273
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunoprecipitation, flow cytometry
product information
AntibodyID :
34781
AntibodyName :
LS-C35273
TargetSpecies :
Human
Host Species :
Goat
Product Name :
CPE / Carboxypeptidase E Antibody (aa42-453) LS-C35273
Specificity :
Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1% cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa42-453
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenDesc :
NS0-derived recombinant human Carboxypeptidase E (aa42-453).
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS.
IsotypeName :
IgG
Gene :
CPE / Carboxypeptidase E
StandardGeneSymbol :
CPE
gene family :
Exopeptidase
Subfamily :
Metallopeptidase M14
Reactivity :
Human
Usage :
ELISA (0.1 - 0.2 µg/ml), Flo, IP, WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
Carboxypeptidase E antibody LS-C35273 is an unconjugated goat polyclonal antibody to human Carboxypeptidase E (CPE) (aa42-453). Validated for ELISA, Flow, IP and WB.
UsageText :
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 ug/ml. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 ug/ml. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/mL and add 10uL to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.
Synonyms :
CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.