This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CDH6 / K Cadherin Antibody (Extracellular Domain) LS-C35232
catalog :
LS-C35232
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, flow cytometry
product information
AntibodyID :
34740
AntibodyName :
LS-C35232
TargetSpecies :
Human
Host Species :
Sheep
Product Name :
CDH6 / K Cadherin Antibody (Extracellular Domain) LS-C35232
Specificity :
Recognizes human Cadherin-6. In direct ELISAs and Western Blot, shows less than 5% cross-reactivity with rhCadherin-8, rhCadherin-11, and rhCadherin-12.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Extracellular Domain
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenDesc :
NS0-derived rhCadherin-6 extracellular domain.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: -20°C; Long term: -20°C.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS.
IsotypeName :
IgG
Gene :
CDH6 / K Cadherin
StandardGeneSymbol :
CDH6
gene family :
Cadherin
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), Flo (50 µg/ml), ICC (10 µg/ml), WB
ShortWebDescription :
K Cadherin antibody LS-C35232 is an unconjugated sheep polyclonal antibody to human K Cadherin (CDH6) (Extracellular Domain). Validated for ELISA, Flow, ICC and WB.
UsageText :
Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Flow Cytometry: 50 ug/ml Add 10 ul of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well.
Synonyms :
CDH6, K Cadherin, K-cadherin, CAD6, Cadherin-6, Fetal kidney cadherin, KCAD, Kidney cadherin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.