antibody

JCHAIN / Ig J Chain Antibody (FITC) LS-C346940

LS-C346940

polyclonal

domestic rabbit

FITC

immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

LS-C346940

Human

Rabbit

JCHAIN / Ig J Chain Antibody (FITC) LS-C346940

Human J chain of dimeric IgA.

Polyclonal

FITC

Lyophilized from PBS, pH 7.2, No preservatives added

Purified protein

Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Fr

Purified IgG

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

1 ml Distilled water

JCHAIN / Ig J Chain

JCHAIN

Human

ELISA, ICC, IF, IHC, IHC-Fr

Ig J Chain antibody LS-C346940 is an FITC-conjugated rabbit polyclonal antibody to human Ig J Chain (JCHAIN). Validated for ELISA, ICC, IF and IHC.

In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

JCHAIN, IGCJ, Immunoglobulin J chain, IGJ, IgJ chain, JCH

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