antibody

Complement C4d Antibody (clone 10-11) LS-C27178

LS-C27178

monoclonal

mouse

nonconjugated

10-11

The same clone is also sold as:

• Invitrogen: MA1-83082
• Bio-Rad: 2222-8004
• OriGene: BM345

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

LS-C27178

Human

Mouse

Complement C4d Antibody (clone 10-11) LS-C27178

Recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR]. C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4ba€™s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ba€™s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Is used to detect the biomarker C4d which has been described as a a€œfootprinta€ of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis.

Monoclonal

10-11

Unconjugated

BBS, 0.09% Sodium Azide

Native, from human plasma.

Protein A purified

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

IgG1

Complement C4d

Human

Rabbit, Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Cat

ELISA (1:5000 - 1:20000), IF (1:250 - 1:600), IHC, IHC-Fr (1:100 - 1:750), IHC-P, WB

Complement C4d antibody LS-C27178 is an unconjugated mouse monoclonal antibody to human Complement C4d. Validated for ELISA, IF, IHC and WB.

Immunohistochemistry: It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6).

Worldwide