This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
POLR2A / RNA polymerase II Antibody LS-C26239
catalog :
LS-C26239
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section
product information
AntibodyID :
26821
AntibodyName :
LS-C26239
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
POLR2A / RNA polymerase II Antibody LS-C26239
Specificity :
Excellent for detection of transcription activity, e. g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200kD band of non-phosphorylated and 240kD band of phosphorylated Pol IIA. Reactivities on Cultured Cell Lines (tested so far): Drosophila chromosomes, PTK-2, HeLa, Hep2. Epitope recognized: 794DDYGPESRGFVENSYLA822. Species cross-reactivity: Human, insects.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.09% Sodium Azide
ImmunogenDesc :
RNA polymerase II. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IsotypeName :
IgG1
Gene :
POLR2A / RNA polymerase II
StandardGeneSymbol :
POLR2A
Reactivity :
Xenopus, Zebrafish, Insect, Drosophila, Horse, Rabbit, Mouse, Dog, Bovine, Bat, Rat, Hamster, Human, Monkey
Usage :
IHC, IHC-Fr, WB
ShortWebDescription :
RNA polymerase II antibody LS-C26239 is an unconjugated mouse monoclonal antibody to RNA polymerase II (POLR2A) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
UsageText :
Suitable for use in Western Blot (Western) and Immunohistochemistry (frozen tissue). Immunohistochemistry: 1:100 (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min). Incubation Time 1 hr at RT.
Synonyms :
POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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