This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
RPS6KB1 / P70S6K / S6K Antibody (aa26-43) LS-C25941
catalog :
LS-C25941
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunoprecipitation
product information
AntibodyID :
26523
AntibodyName :
LS-C25941
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
RPS6KB1 / P70S6K / S6K Antibody (aa26-43) LS-C25941
Specificity :
Recognizes p70 S6 kinase Mr ~64kD. In some cell lysates, an additional protein may be detected at a higher molecular weight. Cross-reacts with rat, human and mouse.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa26-43
PresentationDesc :
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to residues 26-43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
RPS6KB1 / P70S6K / S6K
StandardGeneSymbol :
RPS6KB1
gene family :
Protein Kinase
Subfamily :
P70 RSK
Reactivity :
Mouse, Rat, Human
Usage :
Func, IP, WB
ShortWebDescription :
S6K antibody LS-C25941 is an unconjugated rabbit polyclonal antibody to S6K (RPS6KB1 / P70S6K) (aa26-43) from rat. It is reactive with human, mouse and rat. Validated for Func, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 0.5-2.0 ug/ml detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates p70 S6 kinase from 500 ug of HEK293 RIPA lysate. Immunoprecipitation Kinase Assay: 5 ug immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1 uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.
Synonyms :
RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.