This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NOS1 / nNOS Antibody (aa724-739) LS-C25783
catalog :
LS-C25783
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
product information
AntibodyID :
26365
AntibodyName :
LS-C25783
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
NOS1 / nNOS Antibody (aa724-739) LS-C25783
Specificity :
Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa724-739
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide, 0.1% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Ved in human, rat and mouse. IMMUNOGEN: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.
PurificationDesc :
Affinity purified
IsotypeName :
IgG
Gene :
NOS1 / nNOS
StandardGeneSymbol :
NOS1
Reactivity :
Human
Usage :
ELISA, IF, IHC, WB (1 - 10 µg/ml)
ShortWebDescription :
NNOS antibody LS-C25783 is an unconjugated rabbit polyclonal antibody to human nNOS (NOS1) (aa724-739). Validated for ELISA, IF, IHC and WB.
UsageText :
Immunohistochemistry: Use at 5-10 ug/ml on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4?C with a 1:100-1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1-10 ug/ml with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0 ug/ml or lower on plates coated with 50-100 ng of peptide antigen.
Synonyms :
NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.