This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NES / Nestin Antibody LS-C25743
catalog :
LS-C25743
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
product information
AntibodyID :
26325
AntibodyName :
LS-C25743
TargetSpecies :
Rat
Host Species :
Mouse
Product Name :
NES / Nestin Antibody LS-C25743
Specificity :
Recognizes rodent Nestin at 200-220kD. Specificity confirmed with mouse and rat. Does not cross-react with human.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Nestin purified from embryonic rat spinal cord.
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
NES / Nestin
StandardGeneSymbol :
NES
Reactivity :
Mouse, Rat
Usage :
ICC (1:20 - 1:200), IHC, IHC-Fr (1:10 - 1:100), WB
UsageNotRecommended :
IHC-P
ShortWebDescription :
Nestin antibody LS-C25743 is an unconjugated mouse monoclonal antibody to Nestin (NES) from rat. It is reactive with mouse and rat. Validated for ICC, IHC and WB.
UsageText :
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry (frozen or paraffin): 1:20-1:200. Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM. Immunocytochemistry: 1:20-1:200. Fix cells in 4% paraformaldehyde buffered with 50 mM sodium borate, pH 9.5. Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70?C will show breakdown of nestin sometimes into multiple fragments. Positive control: Mouse embryonic brain tissue.
Synonyms :
NES, Nestin, Nbla00170
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.