This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
MIF Antibody (aa2-32) LS-C25619
catalog :
LS-C25619
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
26201
AntibodyName :
LS-C25619
TargetSpecies :
Human
Host Species :
Chicken
Product Name :
MIF Antibody (aa2-32) LS-C25619
Specificity :
Recognizes human mature Migration Inhibitory Factor, Macrophage (MIF) at 12.3kD. The MIF gene encodes a protein of 115aa. The initiating methionine is cleaved leaving a mature protein of 114aa.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa2-32
PresentationDesc :
Lyophilized from PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
Eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). Peptide sequence: PMFIVNTNVPRASVPDGF- LSELTQQLAQATG. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Pig (94%); Sheep, Panda, Bovine, Horse, Rabbit (89%); Hamster (83%).
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute using sterile distilled H2O.
IsotypeName :
IgY
Gene :
MIF
StandardGeneSymbol :
MIF
Reactivity :
Human, Monkey
Usage :
ELISA (1:1000 - 1:2000), WB (1:500 - 1:1000)
ShortWebDescription :
MIF antibody LS-C25619 is an unconjugated chicken polyclonal antibody to MIF (aa2-32) from human. It is reactive with human and monkey. Validated for ELISA and WB.
UsageText :
Suitable for use in ELISA and Western Blot. Western Blot: 1:500-1:1000. Detects human MIF from supernatants or lysates of 2x1E6 endotoxin-stimulated PBMC stimulated for 24 hours with 1% human serum plus 10 mg/ml E. coli LPS. ELISA: 1:1000-1:2000. Assayed against goat anti-chicken IgG (H&L) in a standard capture ELISA using ABTS as a substrate for 30 min at RT.
Synonyms :
MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.