This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Integrin AlphaV Beta5 Antibody LS-C24771
catalog :
LS-C24771
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunocytochemistry, immunoprecipitation, flow cytometry
product information
AntibodyID :
25353
AntibodyName :
LS-C24771
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Integrin AlphaV Beta5 Antibody LS-C24771
Specificity :
Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, No preservatives added
ImmunogenType :
Purified protein
ImmunogenDesc :
Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
PurificationDesc :
Purified
IsotypeName :
IgG2a
Gene :
Integrin AlphaV Beta5
Reactivity :
Human
Usage :
ELISA, Flo, ICC, IP
ShortWebDescription :
Integrin AlphaV Beta5 antibody LS-C24771 is an unconjugated mouse monoclonal antibody to human Integrin AlphaV Beta5. Validated for ELISA, Flow, ICC and IP.
UsageText :
Immunoprecipitation. Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X105 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody. Immunocytochemistry: 15 mg/ml dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody. ELISA.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.