This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FZD3 / Frizzled 3 Antibody (Biotin) LS-C23709
catalog :
LS-C23709
clonality :
monoclonal
host :
rat
conjugate :
biotin
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
product information
AntibodyID :
24291
AntibodyName :
LS-C23709
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
FZD3 / Frizzled 3 Antibody (Biotin) LS-C23709
Specificity :
Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.
ClonalityDesc :
Monoclonal
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from PBS, BSA
ImmunogenDesc :
NS0-derived rmFrizzled-3 extracellular CRD.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with 1 ml sterile PBS.
IsotypeName :
IgG2a
Gene :
FZD3 / Frizzled 3
StandardGeneSymbol :
FZD3
gene family :
GPCR
Subfamily :
Frizzled
Reactivity :
Mouse, Human
Usage :
Flo, ICC, IHC, IHC-Fr, WB (1 - 2 µg/ml)
ShortWebDescription :
Frizzled 3 antibody LS-C23709 is a biotin-conjugated rat monoclonal antibody to Frizzled 3 (FZD3) from mouse. It is reactive with human and mouse. Validated for Flow, ICC, IHC and WB.
UsageText :
Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 ug/ml to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.