This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FZD3 / Frizzled 3 Antibody LS-C23708
catalog :
LS-C23708
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
product information
AntibodyID :
24290
AntibodyName :
LS-C23708
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
FZD3 / Frizzled 3 Antibody LS-C23708
Specificity :
Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 or -8. Species cross-reactivity: Human.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenDesc :
NS0-derived rmFrizzled-3 extracellular CRD domain.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
ResuspensionLiquidDesc :
Reconstitute in 200 ul sterile PBS
IsotypeName :
IgG2a
Gene :
FZD3 / Frizzled 3
StandardGeneSymbol :
FZD3
gene family :
GPCR
Subfamily :
Frizzled
Reactivity :
Mouse, Human
Usage :
ELISA, Flo, IHC, IHC-Fr, WB
ShortWebDescription :
Frizzled 3 antibody LS-C23708 is an unconjugated rat monoclonal antibody to Frizzled 3 (FZD3) from mouse. It is reactive with human and mouse. Validated for ELISA, Flow, IHC and WB.
UsageText :
Suitable for use in Direct ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. By Flow Cytometry and Immunohistochemistry, this antibody detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Flow Cytometry: Dilute this antibody to 5-10 ug/ml and add 10 ml of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: A biotin conjugate of this antibody at a concentration of 8 ug/ml was used to detect Frizzled-3 in frozen mouse embryo sections. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-3. Using a colorimetric detection system, the detection limit for rmFrizzled-3 is ~1 ng/lane under non-reducing and reducing conditions.
Synonyms :
FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.