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product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
SLC6A3 / Dopamine Transporter Antibody (N-Terminus) LS-C22950
catalog :
LS-C22950
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - frozen section
product information
AntibodyID :
23532
AntibodyName :
LS-C22950
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
SLC6A3 / Dopamine Transporter Antibody (N-Terminus) LS-C22950
Specificity :
Recognizes Dopamine transporter (DAT). Species cross-reactivity: Rat and mouse. Weakly reacts with human and canine.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG
Gene :
SLC6A3 / Dopamine Transporter
StandardGeneSymbol :
SLC6A3
gene family :
Transporter
Subfamily :
Neurotransmitter:sodium symporter
Reactivity :
Mouse, Rat
Usage :
ELISA (0.5 - 1 µg/ml), IHC, IHC-Fr, IP, WB (1 - 10 µg/ml)
ShortWebDescription :
Dopamine Transporter antibody LS-C22950 is an unconjugated rabbit polyclonal antibody to Dopamine Transporter (SLC6A3) (N-Terminus) from rat. It is reactive with mouse and rat. Validated for ELISA, IHC, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and ELISA. Western Blot: 1-10 ug/ml using ECL. An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD. Immunoprecipitation: 2-10 ug. Immunohistochemistry: 2-10 ug/ml on frozen sections of tissues fixed with 4% paraformaldehyde. ELISA: 0.5-1.0 ug/ml using 1 ug/ml of DAT control peptide per well.
Synonyms :
SLC6A3, DA transporter, DAT1, DAT
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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