This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
DSC1 / Desmocollin 1 Antibody (Intracellular) LS-C22805
catalog :
LS-C22805
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
23387
AntibodyName :
LS-C22805
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
DSC1 / Desmocollin 1 Antibody (Intracellular) LS-C22805
Specificity :
Antigen recognized in species specific for human. Cell Lines (tested so far) of differentiated cells. All simple epithelia are negative. desmocollin 1; negative with bovine (tested so far) and rodent material. Reacts with 111kD (form 'a') and 103kD (form 'b') polypeptides (desmocollin1 splice forms, both at pI 5.2) from human epidermal desmosomes. Reactivities on Cultured Positive on primary keratinocytes, however, restricted to local piles.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
Intracellular
PresentationDesc :
Lyophilized PBS, pH 7.4, 0.09% Sodium Azide, 0.5% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC). Percent identity by BLAST analysis: Human, Gibbon, Monkey (96%); Gorilla (92%); Marmoset (88%).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
ResuspensionLiquidDesc :
Reconstitute with 1.0 ml sterile ddH2O
IsotypeName :
IgG1
Gene :
DSC1 / Desmocollin 1
StandardGeneSymbol :
DSC1
gene family :
Cadherin
Reactivity :
Human
Usage :
IHC, IHC-Fr, IHC-P, WB
ShortWebDescription :
Desmocollin 1 antibody LS-C22805 is an unconjugated mouse monoclonal antibody to human Desmocollin 1 (DSC1) (Intracellular). Validated for IHC and WB.
UsageText :
Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry (Frozen, Paraffin, HEIR): 1:10 in PBS, pH 7.4; Incubation Time 1 hr at RT or overnight at 4?C. Note: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended (see ref. 2). Without detergent pretreatment a cytoplasmic component might cross-react (e. g. in fibroblasts). (Paraffin: slight cross-reactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step. Localization: Localizes Dsc1 present in suprabasal layers of interfollicular epidermis; specific cell layers (e. g. in hair follicle root sheath); Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).
Synonyms :
DSC1, Desmocollin-1, Desmocollin 1, Desmosomal glycoprotein 2/3, DG2/DG3, Cadherin family member 1, CDHF1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.