This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CREB1 / CREB Antibody (phospho-Ser133) LS-C22424
catalog :
LS-C22424
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
23006
AntibodyName :
LS-C22424
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
CREB1 / CREB Antibody (phospho-Ser133) LS-C22424
Specificity :
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kD proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide. Species cross-reactivity: Rat, mouse, human and hamster.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer133
PresentationDesc :
0.1 M Tris-glycine, pH 7.4. 150 mM sodium chloride, 0.05% sodium azide
ImmunogenType :
Synthetic phospho-peptide
ImmunogenDesc :
Synthetic phosphopeptide corresponding to residues 123-136 [KRREILSRRP(pS)Y-RK] of rat CREB (cAMP response element binding protein) coupled to keyhole limpet hemocyanin (KLH). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Rabbit, Pig, Turkey, Chicken, Lizard, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG
Gene :
CREB1 / CREB
StandardGeneSymbol :
CREB1
gene family :
Transcription factor
Reactivity :
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Sheep, Bovine, Rabbit, Mouse, Dog, Rat, Hamster, Human, Monkey
Usage :
GS, IHC, IHC-P, IP, WB (1:500)
ShortWebDescription :
CREB antibody LS-C22424 is an unconjugated rabbit polyclonal antibody to CREB (CREB1) (pSer133) from rat. It is reactive with human, mouse, rat and other species. Validated for GS, IHC, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (formalin-fixed, paraffin). Western Blot: 1:500. Detected phosphorylated CREB in RIPA lysates from mouse 3T3 cells treated with 50 mM forskolin. PC-12 and forskolin treated PC-12 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Gel Shift: This antibody has been shown to gel shift. Immunoprecipitation: 20 ul immunoprecipitates phosphorylated CREB from 500 ug of rat PC-12 cells treated with 50 uM forskolin. Immunohistochemistry: 1:500 dilution detects phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.
Synonyms :
CREB1, CREB-1, Transactivator protein, CREB
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.