product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
CSPG4 / NG2 Antibody (Azide-free) LS-C22113
catalog :
LS-C22113
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunocytochemistry, immunoprecipitation, flow cytometry
citations: 1
Reference
Wishart A, Conner S, Guarin J, Fatherree J, Peng Y, McGinn R, et al. Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis. Sci Adv. 2020;6: pubmed publisher
product information
AntibodyID :
22695
AntibodyName :
LS-C22113
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CSPG4 / NG2 Antibody (Azide-free) LS-C22113
Specificity :
Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.
ClonalityDesc :
Monoclonal
AntibodyModification :
Azide-free Unconjugated
PresentationDesc :
PBS, pH 7.6. No preservative added. Glycerol free.
ImmunogenDesc :
Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IsotypeName :
IgG2a
Gene :
CSPG4 / NG2
StandardGeneSymbol :
CSPG4
Reactivity :
Human
Usage :
Flo, Func, IF, IP
ShortWebDescription :
NG2 antibody LS-C22113 is an unconjugated mouse monoclonal antibody to human NG2 (CSPG4). Validated for Flow, Func, IF and IP. Cited in 1 publication.
UsageText :
Suitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Immunofluorescence Microscopy: Cells in culture. Immunoprecipitation: 1 ug/1E6 cells. FACS analysis: 1-2 ug/1E6 cells. Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
CSPG4, MCSP, MEL-CSPG, MSK16, NG2, HMW-MAA, MCSPG
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.