This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD99 Antibody LS-C21964
catalog :
LS-C21964
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, flow cytometry
product information
AntibodyID :
22546
AntibodyName :
LS-C21964
TargetSpecies :
Human
Host Species :
Goat
Product Name :
CD99 Antibody LS-C21964
Specificity :
Recognizes the human CD99R cell surface antigen.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from Sterile Solution PBS, 5% Trehalose
ImmunogenType :
Cells
ImmunogenDesc :
HPB MLT T cell line.
PurificationDesc :
Ascites
ResuspensionLiquidDesc :
Reconstitute in 100 ul sterile PBS
IsotypeName :
IgG3
Gene :
CD99
StandardGeneSymbol :
CD99
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), Flo, WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
CD99 antibody LS-C21964 is an unconjugated goat polyclonal antibody to human CD99. Validated for ELISA, Flow and WB.
UsageText :
Suitable for use in ELISA, Western Blot, Flow Cytometry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 2 ng/lane under non-reducing and reducing. conditions. Flow Cytometry: Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul, using bEnd.3 cells (Schenkel AR, et al., (2007) Cell Commun. Adhes. 14:227). The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Between steps, wash cells in Flow Cytometry Staining Buffer. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 0.2 ng/well.
Synonyms :
CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 molecule, E2 antigen, HBA71
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.