This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-CCND1 / Cyclin D1 Antibody (C-Terminus, clone SP4) LS-C210597
catalog :
LS-C210597
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
SP4
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
product information
AntibodyID :
219251
AntibodyName :
LS-C210597
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-CCND1 / Cyclin D1 Antibody (C-Terminus, clone SP4) LS-C210597
Specificity :
Human CCND1 / Cyclin D1
ClonalityDesc :
Monoclonal
CloneName :
SP4
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
Tissue culture supernatant in TBS, pH 7.5, 1% BSA, 0.1% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from C-terminus of human cyclin D1.
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Store at 2-8°C. Do not freeze.
IsotypeName :
IgG
Gene :
CCND1 / Cyclin D1
StandardGeneSymbol :
CCND1
Reactivity :
Human
Usage :
IHC-P (1:100), WB (1:25)
UsageText :
IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Breast carcinoma, mantle cell lymphoma. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate.
Synonyms :
CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.