This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-KRT18 / CK18 / Cytokeratin 18 Antibody (clone DC10) LS-C210483
catalog :
LS-C210483
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DC10
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
product information
AntibodyID :
219137
AntibodyName :
LS-C210483
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Anti-KRT18 / CK18 / Cytokeratin 18 Antibody (clone DC10) LS-C210483
Specificity :
Human KRT18 / Cytokeratin 18
ClonalityDesc :
Monoclonal
CloneName :
DC10
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.6, 1% BSA, 0.1% sodium azide
ImmunogenType :
Cells
ImmunogenDesc :
Human breast cancer PMC42 cells.
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Store at 2-8°C. Do not freeze.
IsotypeName :
IgG1
Gene :
KRT18 / CK18 / Cytokeratin 18
StandardGeneSymbol :
KRT18
gene family :
Keratin
Reactivity :
Human
Usage :
IHC-P (1:100), WB (1:25)
UsageText :
IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Breast carcinoma, skin. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: A431 cell lysate.
Synonyms :
KRT18, CK-18, Cytokeratin 18, Cytokeratin-18, K18, Keratin 18, CYK18, Keratin-18
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.