antibody

RFP / Red Fluorescent Protein Antibody (clone 8E5.G7) LS-C209691

LS-C209691

monoclonal

mouse

nonconjugated

8E5.G7

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry

LS-C209691

Mouse

RFP / Red Fluorescent Protein Antibody (clone 8E5.G7) LS-C209691

Monoclonal

8E5.G7

Unconjugated

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Fusion protein

Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Protein A affinity chromatography

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

IgG2a,k

RFP / Red Fluorescent Protein

ELISA (1:75000 - 1:150000), Flo, IF, IP, WB (1:1000 - 1:10000)

Red Fluorescent Protein antibody LS-C209691 is an unconjugated mouse monoclonal antibody to Red Fluorescent Protein (RFP). Validated for ELISA, Flow, IF, IP and WB.

Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Worldwide