antibody

Beta Galactosidase Antibody (HRP) LS-C209239

LS-C209239

polyclonal

domestic rabbit

HRP

western blot, ELISA, immunohistochemistry

LS-C209239

E. coli

Rabbit

Beta Galactosidase Antibody (HRP) LS-C209239

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, and anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Polyclonal

HRP

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Purified protein

Beta Galactosidase (E.coli)

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Reconstitute in 100 µL of deionized water.

IgG

Beta Galactosidase

E. coli

ELISA (1:2000 - 1:5000), IHC (1:250), WB (1:500 - 1:2000)

Beta Galactosidase antibody LS-C209239 is an HRP-conjugated rabbit polyclonal antibody to e. coli Beta Galactosidase. Validated for ELISA, IHC and WB.

Anti-Beta Galactosidase is suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 mu m) to produce free-floating sections. A 1:5000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1:10000 dilution was noted to show no background and to be suitable for double labeling experiments. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

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