This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
AKT3 Antibody (Internal, clone 25F6.F6.D8) LS-C209201
catalog :
LS-C209201
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
25F6.F6.D8

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry
product information
AntibodyID :
217855
AntibodyName :
LS-C209201
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
AKT3 Antibody (Internal, clone 25F6.F6.D8) LS-C209201
Specificity :
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
ClonalityDesc :
Monoclonal
CloneName :
25F6.F6.D8
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
PurificationDesc :
Protein A affinity chromatography
RecommendedStorageDesc :
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
IsotypeName :
IgG1,k
Gene :
AKT3
StandardGeneSymbol :
AKT3
gene family :
Protein Kinase
Subfamily :
RAC/AKT
Reactivity :
Human
Usage :
ELISA (1:2000 - 1:10000), Flo, IHC (20 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
AKT3 antibody LS-C209201 is an unconjugated mouse monoclonal antibody to human AKT3 (Internal). Validated for ELISA, Flow, IHC and WB.
UsageText :
Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.