This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Ubiquitin Antibody (clone Ubi-1-AP) LS-C204828
catalog :
LS-C204828
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Ubi-1-AP
application :
western blot, immunohistochemistry, immunocytochemistry
product information
AntibodyID :
213395
AntibodyName :
LS-C204828
TargetSpecies :
Mammal
Host Species :
Mouse
Product Name :
Ubiquitin Antibody (clone Ubi-1-AP) LS-C204828
Specificity :
Clone Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that clone Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. The antibody also works on paraffin embedded material. clone Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. clone Ubi-1 will recognize human, bovine, chicken, Drosophila, and C. elegans in ELISA. clone Ubi-1 also works on Western blots and can be used to study ubiquitinated proteins. It has also been successfully used in competitive ELISA.
ClonalityDesc :
Monoclonal
CloneName :
Ubi-1-AP
AntibodyModification :
Unconjugated
PresentationDesc :
Liquid
ImmunogenType :
Purified protein
ImmunogenDesc :
Purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. MCA-Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
Ubiquitin
Reactivity :
Human
Usage :
IF (1:500), IHC (1:1000), WB
ShortWebDescription :
Ubiquitin antibody LS-C204828 is an unconjugated mouse monoclonal antibody to human Ubiquitin. Validated for IF, IHC and WB.
UsageText :
Ubi-1 is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. We recommend a starting dilution of 1:1000 for this purposes, using ABC or other enzymatic amplification procedures. For immunofluorescence try a dilution of 1:500.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.