product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
mCherry Antibody LS-C204826
catalog :
LS-C204826
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Róna G, Roberti D, Yin Y, Pagan J, Homer H, Sassani E, et al. PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. elife. 2018;7: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C204826 image 1
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, in the lane marked plus. HEK293 cells which were not transfected with this vector show no protein band in lane marked minus.
image 2 :
LifeSpan Biosciences LS-C204826 image 2
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
product information
AntibodyID :
213393
AntibodyName :
LS-C204826
TargetSpecies :
Discosoma
Host Species :
Rabbit
Product Name :
mCherry Antibody LS-C204826
Specificity :
mCherry
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
10 mM Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant full length His tagged mCherry purified from E. coli.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Gene :
mCherry
Usage :
ICC (1:500), IF (1:500), WB (1:1000)
ShortWebDescription :
MCherry antibody LS-C204826 is an unconjugated rabbit polyclonal antibody to mCherry from discosoma. Validated for ICC, IF and WB. Cited in 1 publication.
UsageText :
Try at dilutions of 1:500 and higher for immunofluorescence. For western blots try at 1:1000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.