product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
mCherry Antibody LS-C204825
catalog :
LS-C204825
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 5
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; rat
Augur I, Wyckoff A, Aston Jones G, Kalivas P, Peters J. Chemogenetic Activation of an Extinction Neural Circuit Reduces Cue-Induced Reinstatement of Cocaine Seeking. J Neurosci. 2016;36:10174-80 pubmed publisher
Heinsbroek J, Giannotti G, Mandel M, Josey M, Aston Jones G, James M, et al. A common limiter circuit for opioid choice and relapse identified in a rodent addiction model. Nat Commun. 2021;12:4788 pubmed publisher
Giannotti G, Heinsbroek J, Yue A, Deisseroth K, Peters J. Prefrontal cortex neuronal ensembles encoding fear drive fear expression during long-term memory retrieval. Sci Rep. 2019;9:10709 pubmed publisher
Giannotti G, Barry S, Siemsen B, Peters J, McGinty J. Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking. J Neurosci. 2018;38:8956-8966 pubmed publisher
Kitano M, Hosmillo M, Emmott E, Lu J, Goodfellow I. Selection and Characterization of Rupintrivir-Resistant Norwalk Virus Replicon Cells In Vitro. Antimicrob Agents Chemother. 2018;62: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C204825 image 1
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
image 2 :
LifeSpan Biosciences LS-C204825 image 2
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
product information
AntibodyID :
213392
AntibodyName :
LS-C204825
TargetSpecies :
Discosoma
Host Species :
Chicken
Product Name :
mCherry Antibody LS-C204825
Specificity :
mCherry
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
5 mM Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant full length His tagged mCherry purified from E. coli.
PurificationDesc :
IgY fraction
RecommendedStorageDesc :
Store at 4°C.
IsotypeName :
IgY
Gene :
mCherry
Usage :
ICC (1:1000), IF (1:1000), WB (1:2000)
ShortWebDescription :
MCherry antibody LS-C204825 is an unconjugated chicken polyclonal antibody to mCherry from discosoma. Validated for ICC, IF and WB. Cited in 2 publications.
UsageText :
Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:2000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.