product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
Vimentin Antibody LS-C204593
catalog :
LS-C204593
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:200; loading ...; fig s9c
Crippa S, Rossella V, Aprile A, Silvestri L, Rivis S, Scaramuzza S, et al. Bone marrow stromal cells from β-thalassemia patients have impaired hematopoietic supportive capacity. J Clin Invest. 2019;129:1566-1580 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C204593 image 1
Western blot of crude extract of human embryonic kidney Hek293 cells stained with Vimentin antibody, showing a single strong clean band at ~50kDa.
image 2 :
LifeSpan Biosciences LS-C204593 image 2
View of mixed neuron/glial cultures stained with Vimentin antibody (green) and rabbit antibody to GFAP antibody (red). Vimentin is the sole cytoplasmic intermediate filament subunit expressed in fibroblastic, microglial and endothelial cells. The flattened cells in the middle of the image which appear green are fibroblasts. Astrocytes may express primarily GFAP, or a mix of GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
product information
AntibodyID :
213143
AntibodyName :
LS-C204593
TargetSpecies :
Human
Host Species :
Chicken
Product Name :
Vimentin Antibody LS-C204593
Specificity :
Human Vimentin
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Liquid
ImmunogenType :
Recombinant protein
ImmunogenDesc :
The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgY
Gene :
Vimentin
StandardGeneSymbol :
VIM
Reactivity :
Human
Usage :
ICC (1:5000), IF (1:5000), WB
ShortWebDescription :
Vimentin antibody LS-C204593 is an unconjugated chicken polyclonal antibody to human Vimentin. Validated for ICC, IF and WB.
UsageText :
The titre of this material is about 1:5000 in immunofluorescence microscopy.
Synonyms :
VIM, Vimentin, vimenin
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.