This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FBL / FIB / Fibrillarin Antibody (clone 38F3) LS-C204517
catalog :
LS-C204517
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
38F3

The same clone is also sold as:
reactivity :
human
application :
western blot, immunocytochemistry
product information
AntibodyID :
213067
AntibodyName :
LS-C204517
TargetSpecies :
Yeast
Host Species :
Mouse
Product Name :
FBL / FIB / Fibrillarin Antibody (clone 38F3) LS-C204517
Specificity :
This clone stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei. Subsequently it was found that this antibody recognizes fibrillarin in a wide variety of species including human, rat, Drosophila, S. pombe, C. elegans, and plants. It can therefore be used to identify nucleoli immunocytochemically.
ClonalityDesc :
Monoclonal
CloneName :
38F3
AntibodyModification :
Unconjugated
PresentationDesc :
Tissue Culture Supernatant, 10 mM Sodium Azide
ImmunogenDesc :
Mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
FBL / FIB / Fibrillarin
StandardGeneSymbol :
FBL
Reactivity :
Rat, Human
Usage :
ICC (1:1000 - 1:5000), IF (1:1000 - 1:5000), WB (1:2000 - 1:10000)
ShortWebDescription :
Fibrillarin antibody LS-C204517 is an unconjugated mouse monoclonal antibody to Fibrillarin (FBL / FIB) from yeast. It is reactive with human and rat. Validated for ICC, IF and WB.
UsageText :
For western blots of yeast protein samples, use FBL / FIB / Fibrillarin antibody diluted 1:2000 (cell lysates) to 1:10000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try the antibody diluted 1:1000-1:5000. To detect mammalian fibrillarin on western blots by ECL, try the antibody at 1:500 dilution. For immunofluorescence on yeast cells, use the antibody diluted 1:1000-1:5000. For IF of mammalian cells, try the antibody at 1:500.
Synonyms :
FBL, FLRN, FBRL, FIB1, Fibrillarin, RNU3IP1, FIB
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.