This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ADRA2A Antibody LS-C20327
catalog :
LS-C20327
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
immunohistochemistry, immunocytochemistry
product information
AntibodyID :
20909
AntibodyName :
LS-C20327
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
ADRA2A Antibody LS-C20327
Specificity :
Recognizes rat alpha 2a Adrenergic Receptor. Shows positive staining in rat spinal cord dorsal horn, ventral horn motor neurons and retina. When examined in other brain regions known to express alpha 2a, staining was negative. Species cross-reactivity: Mouse and human.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
ImmunogenDesc :
(C)KASRWRGRGNREKR; Cysteine added for conjugation. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Panda, Cat, Bovine, Pig, Opossum, Guinea pig, Lizard (93%); Pufferfish, Zebrafish (86%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG
Gene :
ADRA2A
StandardGeneSymbol :
ADRA2A
gene family :
GPCR
Subfamily :
Adrenergic
Reactivity :
Rat
Usage :
ICC, IF, IHC (1:100 - 1:500)
ShortWebDescription :
ADRA2A antibody LS-C20327 is an unconjugated rabbit polyclonal antibody to rat ADRA2A. Validated for ICC, IF and IHC.
UsageText :
Suitable for use in Immunohistochemistry and Immunocytochemistry. Immunohistochemistry: 1:100-1:500; Antiserum was used on perfusion fixed tissue. Perfusion: 1) Calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and cover-slipped. Staining was examined using fluorescence microscopy.
Synonyms :
ADRA2A, Alpha-2A adrenoreceptor, Alpha-2AAR, Alpha-2AAR subtype C10, ADRA2, ADRA2R, ADRAR, Adrenoceptor alpha 2A, Alpha 2a, Alpha(2A)-AR, Alpha2a, ALPHA2AAR, Alpha-2A adrenoceptor, Adrenergic alpha 2a receptor, Adrenergic alpha 2a receptor, Alpha-2A adrenergic receptor
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.