This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NOTCH1 Antibody (N-Terminus) LS-C19109
catalog :
LS-C19109
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
19857
AntibodyName :
LS-C19109
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
NOTCH1 Antibody (N-Terminus) LS-C19109
Specificity :
This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
NOTCH1
StandardGeneSymbol :
NOTCH1
Reactivity :
Mouse, Rat, Human
Usage :
ELISA (1:20000 - 1:60000), IHC (1:200), WB (1:500 - 1:2000)
ShortWebDescription :
NOTCH1 antibody LS-C19109 is an unconjugated rabbit polyclonal antibody to NOTCH1 (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 3 publications.
UsageText :
This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide. This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kD band corresponding to Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature.
Synonyms :
NOTCH1, Notch 1, TAN1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.