This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
AKT2 Antibody (aa450-475) LS-C19061
catalog :
LS-C19061
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, flow cytometry
product information
AntibodyID :
19809
AntibodyName :
LS-C19061
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
AKT2 Antibody (aa450-475) LS-C19061
Specificity :
The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa450-475
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near aa 450-475 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
IsotypeName :
IgG
Gene :
AKT2
StandardGeneSymbol :
AKT2
gene family :
Protein Kinase
Subfamily :
RAC/AKT
Reactivity :
Human
Usage :
ELISA (1:4000 - 1:16000), Flo, WB (1:1000)
ShortWebDescription :
AKT2 antibody LS-C19061 is an unconjugated rabbit polyclonal antibody to human AKT2 (aa450-475). Validated for ELISA, Flow and WB. Cited in 1 publication.
UsageText :
This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1000 dilution showing a band at approximately 60 kD. A working dilution of 1:4000 to 1:16000 is suggested for this product in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.
Synonyms :
AKT2, PKB beta, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, Rac protein kinase beta, RAC-BETA, HIHGHH, PKBB
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.