This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C18942
catalog :
LS-C18942
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8G1

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
product information
AntibodyID :
19690
AntibodyName :
LS-C18942
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C18942
ClonalityDesc :
Monoclonal
CloneName :
8G1
AntibodyModification :
Unconjugated
AntigenModification :
aa1-350
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
BUB1B / BubR1
StandardGeneSymbol :
BUB1B
gene family :
Protein Kinase
Subfamily :
BUB1
Reactivity :
Human
Usage :
ELISA (1:5000 - 1:20000), IF (1:200 - 1:1000), IP (1:100), WB (1:200 - 1:1000)
ShortWebDescription :
BubR1 antibody LS-C18942 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B) (aa1-350). Validated for ELISA, IF, IP and WB.
UsageText :
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
Synonyms :
BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.