antibody

NOTCH3 Antibody (clone HMN3-133) LS-C188664

LS-C188664

monoclonal

hamsters

nonconjugated

HMN3-133

human, mouse

LS-C188664

Mouse

Hamster

NOTCH3 Antibody (clone HMN3-133) LS-C188664

Recognizes Notch 3, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 3 is primarily expressed by proliferating neuroepithelium and arterial smooth muscle cells, and may play a role during CNS development. Notch 3 is also present on some thymocytes subsets and Treg cells, and Notch 3 signalling plays a role in mammalian T cell lineage commitment, thymocyte development, and stem cell haematopoiesis. Studies have implicated the over-expression of Notch 3 in T-cell leukaemia. In humans, mutations in the NOTCH3 gene are responsible for the heritable vascular dementia known as CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephelopathy syndrome), predisposing to early onset stroke. Studies have implicated Notch 3 as crucial for ErbB2-negative breast cancer development, and possibly as a therapeutic target for these tumors, which at present lack effective molecular targets. Clone HMN3-133 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

Monoclonal

HMN3-133

Unconjugated

PBS, 0.09% Sodium Azide

Fusion protein

Mouse Notch 3-Fc fusion protein.

Protein G purified

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

IgG

NOTCH3

NOTCH3

Fibronectin

Mouse

Flo

NOTCH3 antibody LS-C188664 is an unconjugated hamster monoclonal antibody to mouse NOTCH3. Validated for Flow.

NOTCH3, CASIL, CADASIL, Notch 3, Notch (Drosophila) homolog 3, Notch homolog 3, Notch homolog 3 (Drosophila)

Worldwide