This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
5-mC / 5-Methylcytosine Antibody (Preservative Free) LS-C187857
catalog :
LS-C187857
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
application :
western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
195760
AntibodyName :
LS-C187857
TargetSpecies :
Broad
Host Species :
Sheep
Product Name :
5-mC / 5-Methylcytosine Antibody (Preservative Free) LS-C187857
Specificity :
Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occurring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, whereas in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements. The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites. Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers. Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.
ClonalityDesc :
Polyclonal
AntibodyModification :
Preservative Free Unconjugated
PresentationDesc :
PBS
ImmunogenType :
Hapten
ImmunogenDesc :
Keyhole limpet hemocyanin (KLH) conjugated 5-methylcytosine.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG
Gene :
5-mC / 5-Methylcytosine
Reactivity :
Broad
Usage :
IF, IHC-Fr (10 µg/ml), IHC-P (10 µg/ml), IP (25 - 100 µg/ml), WB (1:1000)
ShortWebDescription :
5-Methylcytosine antibody LS-C187857 is an unconjugated sheep polyclonal antibody to broad 5-Methylcytosine (5-mC). Validated for IF, IHC, IP and WB.
UsageText :
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.