CDK1 / CDC2 Antibody (clone POH-1) LS-C18772 | LifeSpan Biosciences LS-C18772 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

CDK1 / CDC2 Antibody (clone POH-1) LS-C18772

catalog :

LS-C18772

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

POH-1

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 3

Reference
Palmer A, Gavin A, Nebreda A. A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1. EMBO J. 1998;17:5037-47 pubmed
Ye X, Fincher R, Tang A, McNeal K, Gygax S, Wexler A, et al. Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. J Biol Chem. 1997;272:33384-93 pubmed
Lukas J, Draetta G, Bartek J. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992;207:169-76 pubmed

product information

AntibodyID :

19520

AntibodyName :

LS-C18772

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

CDK1 / CDC2 Antibody (clone POH-1) LS-C18772

Specificity :

Reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.

ClonalityDesc :

Monoclonal

CloneName :

POH-1

AntibodyModification :

Unconjugated

PresentationDesc :

0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide

ImmunogenType :

Recombinant protein

ImmunogenDesc :

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.

PurificationDesc :

Protein A purified

RecommendedStorageDesc :

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

IsotypeName :

IgG2a,k

Gene :

CDK1 / CDC2

StandardGeneSymbol :

CDK1

gene family :

Protein Kinase

Subfamily :

CDC2/CDK

Reactivity :

Mouse, Rat, Human

Usage :

ELISA (1:5000 - 1:20000), IHC, IHC-P, IP (1:100), WB (1:500 - 1:1000)

ShortWebDescription :

CDC2 antibody LS-C18772 is an unconjugated mouse monoclonal antibody to CDC2 (CDK1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Cited in 3 publications.

UsageText :

This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.

Synonyms :

CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.