antibody

NFYA Antibody (N-Terminus) LS-C18769

LS-C18769

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

LS-C18769

Human

Rabbit

NFYA Antibody (N-Terminus) LS-C18769

Polyclonal

Unconjugated

N-Terminus

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Native protein

Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Delipidation, salt fractionation and ion exchange chromatography

Short term: -20°C; Long term: -70°C.

IgG

NFYA

NFYA

Human

ELISA (1:10000), IHC, IHC-P (5 µg/ml), WB (1:1000)

NFYA antibody LS-C18769 is an unconjugated rabbit polyclonal antibody to human NFYA (N-Terminus). Validated for ELISA, IHC and WB.

Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the differentially spliced 35 and 42 kD forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. No reaction was observed by immunoblot against the 25 kD B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 ul per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:10000 is suggested from this experiment. No reactivity was observed by ELISA against the B subunit of NF-Y.

NFYA, CBF-A, CBF-B, HAP2, HAP2 CCAAT-binding protein, NF-YA

Worldwide