This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NFYB Antibody (N-Terminus) LS-C18766
catalog :
LS-C18766
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
19514
AntibodyName :
LS-C18766
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
NFYB Antibody (N-Terminus) LS-C18766
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Native protein
ImmunogenDesc :
NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
PurificationDesc :
Delipidation, salt fractionation and ion exchange chromatography
RecommendedStorageDesc :
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
NFYB
StandardGeneSymbol :
NFYB
Reactivity :
Human
Usage :
ELISA (1:5000 - 1:25000), GS, WB (1:500 - 1:3000)
ShortWebDescription :
NFYB antibody LS-C18766 is an unconjugated rabbit polyclonal antibody to human NFYB (N-Terminus). Validated for ELISA, GS and WB.
UsageText :
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the 25 kD B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NF-Y (13 subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (13 subunit specific). Minimal reaction was observed by immunoblot against the 35 kD A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 ul per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.
Synonyms :
NFYB, CBF-A, CBF-B, HAP3, NF-YB
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.