This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CDKN1A / WAF1 / p21 Antibody LS-C18529
catalog :
LS-C18529
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunoprecipitation
product information
AntibodyID :
19270
AntibodyName :
LS-C18529
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CDKN1A / WAF1 / p21 Antibody LS-C18529
Specificity :
Recognizes WAF1, p21,~21kD. Species cross-reactivity: mouse.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.5% sodium azide.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k + IgG2b,k
Gene :
CDKN1A / WAF1 / p21
StandardGeneSymbol :
CDKN1A
Reactivity :
Mouse, Human
Usage :
IP, WB
ShortWebDescription :
P21 antibody LS-C18529 is an unconjugated mouse monoclonal antibody to p21 (CDKN1A / WAF1) from human. It is reactive with human and mouse. Validated for IP and WB.
UsageText :
Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4 ug immunoprecipitates Cip1 from 500 ug of HeLa nuclear extract. Western Blot Analysis: 0.5-2 ug/ml detects WAF1, p21 in 20 ug of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: HeLa nuclear extract. Use 20 ug per lane for minigels.
Synonyms :
CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.