This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PTPN11 / SHP-2 / NS1 Antibody (aa2-216) LS-C17830
catalog :
LS-C17830
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry, neutralization, immunoprecipitation
product information
AntibodyID :
18571
AntibodyName :
LS-C17830
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
PTPN11 / SHP-2 / NS1 Antibody (aa2-216) LS-C17830
Specificity :
Recognizes murine SHP 2/SHPTP-2 at 64kD. Apparent size increases to ~70kD upon tyrosine phosphorylation. Species cross-reactivity: Human and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa2-216
PresentationDesc :
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 40% glycerol.
ImmunogenType :
Fusion protein
ImmunogenDesc :
GST fusion protein containing aa2-216 of murine SHP 2/SHPTP-2.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
PTPN11 / SHP-2 / NS1
StandardGeneSymbol :
PTPN11
gene family :
Protein Phosphatase
Subfamily :
NT2
Reactivity :
Mouse, Rat, Human
Usage :
ICC (10 µg/ml), IP (1 µg/ml), Neut, WB (1 - 4 µg/ml)
ShortWebDescription :
NS1 antibody LS-C17830 is an unconjugated rabbit polyclonal antibody to NS1 (PTPN11 / SHP-2) (aa2-216) from mouse. It is reactive with human, mouse and rat. Validated for ICC, IP, Neut and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-4 ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts. Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1 ug/ml for this application. Immunoprecipitation: 6 ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells. Immunocytochemistry: 10 ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells. Neutralization: Does not inhibit phosphotyrosine phosphatase activity.
Synonyms :
PTPN11, Bptp-3, BPTP3, CFC, Csw, Corkscrew csw, Gene corkscrew, Phosphatase corkscrew, PTP-2C, SHP2, PTP-1D, PTP2C, SHP-2, SHPTP2, Noonan syndrome 1, SH-PTP2, SH-PTP3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.