This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
MAPK8 / JNK1 Antibody LS-C17753
catalog :
LS-C17753
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunoprecipitation
product information
AntibodyID :
18494
AntibodyName :
LS-C17753
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
MAPK8 / JNK1 Antibody LS-C17753
Specificity :
Recognizes SAPK1a (54kD). Weakly recognizes SAPK1b (54kD). The protein detected with a MWapp of 45kD may be SAPK1c. Species Reactivity: Human, mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.01 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
ImmunogenType :
Fusion protein
ImmunogenDesc :
GST fusion protein corresponding to full-length rat SAPK1a. This sequence is approximately 85% identical to rat SAPK1b and rat SAPK1c. These sequences are highly conserved among mammalian species.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
MAPK8 / JNK1
StandardGeneSymbol :
MAPK8
gene family :
Protein Kinase
Subfamily :
MAPK
Reactivity :
Mouse, Rat, Human
Usage :
Func, IP, WB
ShortWebDescription :
JNK1 antibody LS-C17753 is an unconjugated rabbit polyclonal antibody to JNK1 (MAPK8) from rat. It is reactive with human, mouse and rat. Validated for Func, IP and WB.
UsageText :
Suitable for use in Western Blot analysis and Immunoprecipitation. Western Blot Analysis: 1 ug/ml detected SAPK1 in RIPA lysates from human A431, murine 3T3 and rat PC-12 cells. At 1 ug/ml, the signal detected with 10 ng of gst-SAPK1a was approximately three times greater than the signal detected of with 10 ng gst-SAPK1b. Included Positive Antigen Control: Non-stimulated A431 cell lysate. Use 20 ug per lane for minigels. Immunoprecipitation Protein Kinase Assay: 4 ug immunoprecipitated active recombinant rat SAPK1a, which then catalyzed the transfer of the y-phosphate of [y-32 P]-ATP to c-Jun protein substrate. Western Blot Analysis A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SAPK1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Synonyms :
MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.