This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CDKN1B / p27 Kip1 Antibody (phospho-Ser10) LS-C17121
catalog :
LS-C17121
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation
product information
AntibodyID :
17862
AntibodyName :
LS-C17121
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
CDKN1B / p27 Kip1 Antibody (phospho-Ser10) LS-C17121
Specificity :
Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls. Species cross-reactivity: Human, rat and mouse.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer10
PresentationDesc :
PBS, pH 7.4, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Gene :
CDKN1B / p27 Kip1
StandardGeneSymbol :
CDKN1B
Reactivity :
Mouse, Rat, Human
Usage :
IF (5 - 10 µg/ml), IP, WB (1 - 3 µg/ml)
ShortWebDescription :
P27 Kip1 antibody LS-C17121 is an unconjugated rabbit polyclonal antibody to p27 Kip1 (CDKN1B) (pSer10) from human. It is reactive with human, mouse and rat. Validated for IF, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1-3 ug/ml. Immunoprecipitation: 5-10 ug/IP reaction. Immunofluorescence: 5-10 ug/ml.
Synonyms :
CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.