This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CDKN1B / p27 Kip1 Antibody (aa83-204) LS-C17116
catalog :
LS-C17116
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
17857
AntibodyName :
LS-C17116
TargetSpecies :
Mouse
Host Species :
Mouse
Product Name :
CDKN1B / p27 Kip1 Antibody (aa83-204) LS-C17116
Specificity :
Highly specific for p27kip1 (27kD). Does not cross-react with other closely related mitotic inhibitors. Co-precipitates cdk4 in complex p27Kip1. Its epitope is mapped between aa83-204 of p27. Species cross-reactivity: Human, monkey, canine, mouse and rat. Does not cross-react with bovine.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa83-204
PresentationDesc :
PBS, pH 7.4, 7.5 mM Sodium Azide, 40% Glycerol, 0.1% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Mouse recombinant p27Kip1 protein.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
CDKN1B / p27 Kip1
StandardGeneSymbol :
CDKN1B
Reactivity :
Mouse, Dog, Rat, Human, Monkey
Usage :
Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB (1:100 - 1:200)
ShortWebDescription :
P27 Kip1 antibody LS-C17116 is an unconjugated mouse monoclonal antibody to p27 Kip1 (CDKN1B) (aa83-204) from mouse. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB.
UsageText :
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:100-1:200. Immunohistochemistry: 1:25-1:50 (Frozen, formalin/methacarn, and paraffin embedded sections). Note: Staining of formalin/paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Immunoprecipitation: 10 ul/mg of protein lysate. Positive control: HeLa cells, MAD 109 cells, Ls174T cells, breast carcinoma or colon carcinoma.
Synonyms :
CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.