This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CDKN1A / WAF1 / p21 Antibody (aa58-77) LS-C17111
catalog :
LS-C17111
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
17852
AntibodyName :
LS-C17111
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CDKN1A / WAF1 / p21 Antibody (aa58-77) LS-C17111
Specificity :
Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa58-77
PresentationDesc :
Supplied s a liquid in PBS, pH 7.4, 0.1% sodium azide.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Full length recombinant human p21Cip1/WAF1.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG1,k
Gene :
CDKN1A / WAF1 / p21
StandardGeneSymbol :
CDKN1A
Reactivity :
Human
Usage :
Flo (2 µg/ml), IF (1 - 5 µg/ml), IHC, IHC-Fr, IHC-P, IP, WB
ShortWebDescription :
P21 antibody LS-C17111 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1) (aa58-77). Validated for Flow, IF, IHC, IP and WB.
UsageText :
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 ug/ml. Flow Cytometry: 2 ug/ml. Western Blot (chemiluminescence): 0.1-1 ug/ml. Immunohistochemistry (frozen/paraffin): 5 ug/ml. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 ug/IP reaction. Immunoprecipitates p21 but not associated proteins.
Synonyms :
CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.