This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Osteocalcin Antibody (aa1-20) LS-C17044
catalog :
LS-C17044
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Langen U, Pitulescu M, Kim J, Enriquez Gasca R, Sivaraj K, Kusumbe A, et al. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat Cell Biol. 2017;19:189-201 pubmed publisher
product information
AntibodyID :
17785
AntibodyName :
LS-C17044
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
Osteocalcin Antibody (aa1-20) LS-C17044
Specificity :
Recognizes intracellular N-terminal mouse osteocalcin. Does not cross-react with rat osteocalcin.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-20
PresentationDesc :
Lyophilized, 10mM PBS, pH 7.4, 1% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to aa1-20 (YLGASVPSPDPLEPTREQCE) of intracellular N-Terminus mouse osteocalcin. Percent identity by BLAST analysis: Mouse (100%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
ResuspensionLiquidDesc :
50ul sterile ddH2O, 0.1% sodium azide. Dilute further with 10mM PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
IsotypeName :
IgG
Gene :
Osteocalcin
StandardGeneSymbol :
BGLAP
Reactivity :
Mouse
Usage :
IHC, IHC-P (1 - 2 µg/ml)
UsageNotRecommended :
WB
ShortWebDescription :
Osteocalcin antibody LS-C17044 is an unconjugated rabbit polyclonal antibody to mouse Osteocalcin (aa1-20). Validated for IHC. Cited in 2 publications.
UsageText :
Suitable for use in Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Paraffin sections): 1-2 ug/ml. Trypsin treatment recommended for antigen retrieval. "Osteocalcin was detected on formalin-fixed, paraffin-embedded sections. Briefly, slides were heated at 55°C for 15 min, deparaffinized in two washes of xylene, and rehydrated in a decreasing ethanol gradient. Antigen retrieval was performed using 0.5% trypsin for 30 min at 37°C in a humidified chamber. Endogenous peroxidase was deactivated with 3% H2O2 for 30 min, and sections were blocked in PBS supplemented with 0.1% Triton-X, 2% goat serum, and 1% BSA for 20 min at RT. Sections were incubated with primary antibody (1:200 dilution) in blocking solution overnight at 4°C. Sections were then washed in PBS and incubated with a IgG (HRP) Pab donkey x rabbit secondary antibody in PBS for 45 min at RT. After washing with PBS, HRP activity was detected using a DAB substrate kit according to the manufacturer's instructions.
Synonyms :
BGLAP, Bone Gla protein, BGP, OC, Osteocalcin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.