product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-MTOR Antibody (aa2433-2450) LS-C16621
catalog :
LS-C16621
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
immunoprecipitation, western blot
more info or order :
LifeSpan Biosciences product webpage
product information
?AntibodyID :
17362
AntibodyName :
LS-C16621
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-MTOR Antibody (aa2433-2450) LS-C16621
Specificity :
Recognizes human mTOR, Mr ~240kD. Species cross-reactivity: mouse and rat.
ClonalityDesc :
Polyclonal
AntigenModification :
aa2433-2450
PresentationDesc :
50 mM potassium phosphate, 5 ug/ml BSA, 0.05% sodium azide, 30% glycerol.
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated, synthetic peptide C-DTNAKGNKRSRTRTDSYS corresponding to amino acids 2433-2450 of rat mTOR, mammalian target of rapamycin. mTOR is also known as FRAP or RAFT. Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Goat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (94%); Opossum, Salmon, Stickleback, Zebrafish (89%); Pufferfish (83%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
IsotypeName :
IgG
Gene :
MTOR
StandardGeneSymbol :
MTOR
gene family :
Protein Kinase
Subfamily :
PI3/PI4
Reactivity :
Human, Rat
Usage :
IP, WB
UsageText :
Suitable for use in Western Blot analysis, Immunoprecipitation/Kinase, and mTOR kinase activation. Western Blot Analysis: A 1:500-1:2000 dilution detected mTOR in RIPA lysates from 293T cells transfected with mTOR. 293T cell transfected lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-mTOR (mTAB1) (1:2000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Binding of this antibody is disrupted by phosphorylation that occurs within the immunizing sequence domain. Immunoprecipitation/Kinase: Immunoprecipitates activate mTOR. mTOR Kinase activation: Binding of this antibody has been reported to activate mTOR.
Synonyms :
MTOR, FRAP1, FRAP2, FRAP, Mammalian target of rapamycin, RAFT1, Rapamycin and FKBP12 target 1, RAPT1, Rapamycin target protein 1
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.