product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
MAP1LC3A / LC3A Antibody LS-C165694
catalog :
LS-C165694
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Song D, Lee F. Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. J Biol Chem. 2011;286:15797-805 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C165694 image 1
Fluorescent image of U251 cells stained LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
image 2 :
LifeSpan Biosciences LS-C165694 image 2
Western blot of anti-LC3 antibody HeLa cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
image 3 :
LifeSpan Biosciences LS-C165694 image 3
10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children s Hospital Medical Center.
product information
AntibodyID :
173041
AntibodyName :
LS-C165694
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
MAP1LC3A / LC3A Antibody LS-C165694
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
IsotypeName :
IgG1,k
Gene :
MAP1LC3A / LC3A
StandardGeneSymbol :
MAP1LC3A
Reactivity :
Mouse, Rat, Human
Usage :
IF (1:200), IHC (1:50 - 1:100), WB (1:1000)
ShortWebDescription :
LC3A antibody LS-C165694 is an unconjugated mouse monoclonal antibody to LC3A (MAP1LC3A) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 1 publication.
Synonyms :
MAP1LC3A, ATG8E, LC3, LC3A, MAP1BLC3, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.